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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression <t>saturation</t> binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.
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CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression saturation binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.

Journal: The Journal of Biological Chemistry

Article Title: Polypurine reverse hoogsteen hairpins as a therapeutic tool for SARS-CoV-2 infection

doi: 10.1016/j.jbc.2024.107884

Figure Lengend Snippet: CC1 and CC3 PPRH binding to their corresponding SARS-CoV-2 genome target sequences. A , representative images of the binding assays with CC1 and CC3 PPRHs targeting replicase and spike regions of SARS-CoV-2 and their corresponding constants of dissociation ( Kd ). B , binding curves for RNA-PPRH and DNA-PPRH triplex formation are represented as the mean ± SEM of three independent experiments. Kd s values were calculated after fitting the binding data as nonlinear regression saturation binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.

Article Snippet: The model to fit the binding data was a nonlinear regression saturation binding (One site-specific binding) using the GraphPad Software, Prism v. 9.0.1.

Techniques: Binding Assay, Software